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Article

Challenges to increasing targeting efficiency in genome engineering.

The Journal of reproduction and development - 1 Jan 2016

Horii Takuro, Hatada Izuho

Abstract excerpt

Gene targeting technologies are essential for the analysis of gene functions. Knockout mouse generation via genetic modification of embryonic stem cells (ESCs) is the commonest example, but it is a time-consuming and labor-intensive procedure. Recently, a novel genome editing technology called CRISPR/Cas has enabled the direct production of knockout mice by non-homologous end joining (NHEJ)-mediated mutations....

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Identifiers

PMID: 26688299
DOI: 10.1262/jrd.2015-151
PMCID: PMC4768773

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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