Article
Functional analysis of secreted and transmembrane proteins critical to mouse development.
Nature genetics - 1 Jul 2001
Mitchell K J, Pinson K I, Kelly O G, Brennan J, Zupicich J, Scherz P, Leighton P A, Goodrich L V, Lu X, Avery B J, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes W C
Abstract excerpt
We describe the successful application of a modified gene-trap approach, the secretory trap, to systematically analyze the functions in vivo of large numbers of genes encoding secreted and membrane proteins. Secretory-trap insertions in embryonic stem cells can be transmitted to the germ line of mice with high efficiency and effectively mutate the target gene. Of 60 insertions analyzed in mice, one-third cause...
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