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Article

Real-time PCR based HLA-B*27 screening directly in whole blood.

HLA - 1 Mar 2020

Geiger Kathrin, Zach Christina, Leiherer Andreas, Fraunberger Peter, Drexel Heinz, Muendlein Axel

Abstract excerpt

The linkage between the occurrence of human leucocyte antigen B*27 (HLA-B*27) and ankylosing spondylitis or other related spondyloarthritides is well documented. PCR based methods are widely used for HLA-B*27 screening. To refine HLA-B*27 testing we aimed at establishing a real-time PCR protocol to detect the HLA-B*27 allele directly in blood samples, without DNA extraction. HLA-B*27 analysis was performed by two...

Topics

Alleles
Genes, MHC Class I
HLA-B Antigens
Humans
Real-Time Polymerase Chain Reaction
Reproducibility of Results

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 31749313
DOI: 10.1111/tan.13767

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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