Article
Rapid HLA-B27 screening with real-time TaqMan PCR: a clinical validation in the Dutch population.
Clinical chemistry and laboratory medicine - 6 Sept 2011
Roelandse-Koop Elianne A, Buisman Bert, van Hannen Erik J, van der Zee Anneke, Kortlandt Wouter, Hermans Mirjam H A, van Houte Arend-Jan, van Rhee-Luderer Rianne
Abstract excerpt
BACKGROUND: Human leukocyte antigen B27 (HLA-B27) is strongly associated with ankylosing spondylitis. The B27 allele is present in 90% of patients with this disease, whereas it is present in only 9% of Caucasians. Molecular detection of HLA-B27 is traditionally based on allele specific amplification of exon 2 (Olerup method) or exon 3 (Dominguez method) by PCR, followed by gel analysis. METHODS: We developed a...
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