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Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.

Clinical laboratory - 1 Jan 2001

Tiemann C, Vogel A, Dufaux B, Zimmer M, Krone J R, Hagedorn H J

Abstract excerpt

For clinical diagnostic routine we developed a fast DNA typing of HLA-B27 by PCR and real-time detection using LightCycler technology. The method combines the sensitivity and specificity of PCR with the swiftness of the LightCycler system. The amplification step was performed with a primer set coding for a region in the third exon common to B*2701 to B*2705. The PCR cycles were monitored continuously using the...

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PMID: 11294575

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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