Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Residue 21 of human granulocyte-macrophage colony-stimulating factor is critical for biological activity and for high but not low affinity binding.

The EMBO journal - 1 Mar 1992

Lopez A F, Shannon M F, Hercus T, Nicola N A, Cambareri B, Dottore M, Layton M J, Eglinton L, Vadas M A

Abstract excerpt

The functional role of the predicted first alpha-helix of human granulocyte-macrophage colony-stimulating factor (GM-CSF) was analysed by site-directed mutagenesis and multiple biological and receptor binding assays. Initial deletion mutagenesis pointed to residues 20 and 21 being critical. Subst...

Topics

Amino Acids
Antibody-Dependent Cell Cytotoxicity
Binding, Competitive
Blotting, Western
Cell Line
Chromatography, Affinity
Escherichia coli
Genetic Vectors
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Radioligand Assay

Receptors, Granulocyte-Macrophage Colony-Stimulating Factor

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 1532144
DOI: 10.1002/j.1460-2075.1992.tb05129.x
PMCID: PMC556531

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1532144-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Residue 21 of human granulocyte-macrophage colony-stimulating factor is critical for biological activity and for high but not low affinity binding.PMID 1532144Read ↗

Characterization of the receptor binding determinants of granulocyte colony stimulating factorSemantic proximity Read ↗

The Ig-like domain of human GM-CSF receptor alpha plays a critical role in cytokine binding and receptor activation.Semantic proximity Read ↗

Synthetic alleles at position 121 define a functional domain of human interleukin-1 beta.Semantic proximity Read ↗

Alanine-scanning Mutagenesis of α-Helix D Segment of Interleukin-13 Reveals New Functionally Important Residues of the CytokineSemantic proximity Read ↗

Molecular determinants of the granulocyte-macrophage colony-stimulating factor receptor complex assembly.Semantic proximity Read ↗

Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation.Semantic proximity Read ↗

Human IL-21 and IL-4 bind to partially overlapping epitopes of common gamma-chain.Semantic proximity Read ↗

Human epidermal growth factor. Distinct roles of tyrosine 37 and arginine 41 in receptor binding as determined by site-directed mutagenesis and nuclear magnetic resonance spectroscopy.Semantic proximity Read ↗

Identification of ligand-binding site III on the immunoglobulin-like domain of the granulocyte colony-stimulating factor receptor.Semantic proximity Read ↗

Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor.Semantic proximity Read ↗

Identification of key residues in the amino-terminal third of human interleukin-1 alpha.Semantic proximity Read ↗

Identification of ciliary neurotrophic factor (CNTF) residues essential for leukemia inhibitory factor receptor binding and generation of CNTF receptor antagonists.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.