Article
Single proline substitutions in predicted alpha-helices of murine granulocyte-macrophage colony-stimulating factor result in a loss in bioactivity and altered glycosylation.
The Journal of biological chemistry - 15 Mar 1991
Altmann S W, Johnson G D, Prystowsky M B
Abstract excerpt
Contributions of alpha-helices to biological activity in murine granulocyte-macrophage colony-stimulating factor were analyzed using site-directed mutagenesis and protein expression in COS-1 cells. A series of single proline substitutions were made for residues within the four predicted alpha-hel...
Topics
- Amino Acid Sequence
- Animals
- Electrophoresis, Polyacrylamide Gel
- Glycosylation
- Granulocyte-Macrophage Colony-Stimulating Factor
- Mice
- Molecular Sequence Data
- Mutation
- Proline
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Transfection