Article
Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae.
The Journal of biological chemistry - 15 Oct 2004
Viladevall Laia, Serrano Raquel, Ruiz Amparo, Domenech Gema, Giraldo Jesús, Barceló Anna, Ariño Joaquín
Abstract excerpt
Exposure of the yeast Saccharomyces cerevisiae to alkaline stress resulted in adaptive changes that involved remodeling the gene expression. Recent evidence suggested that the calcium-activated protein phosphatase calcineurin could play a role in alkaline stress signaling. By using an aequorin luminescence reporter, we showed that alkaline stress resulted in a sharp and transient rise in cytoplasmic calcium. This...
Topics
- Calcium
- DNA Primers
- Gene Expression Regulation, Fungal
- Genotype
- Hydrogen-Ion Concentration
- Kinetics
- Polymerase Chain Reaction
- RNA, Fungal
- Saccharomyces cerevisiae
