Article
RPAP1, a novel human RNA polymerase II-associated protein affinity purified with recombinant wild-type and mutated polymerase subunits.
Molecular and cellular biology - 1 Aug 2004
Jeronimo Célia, Langelier Marie-France, Zeghouf Mahel, Cojocaru Marilena, Bergeron Dominique, Baali Dania, Forget Diane, Mnaimneh Sanie, Davierwala Armaity P, Pootoolal Jeff, Chandy Mark, Canadien Veronica, Beattie Bryan K, Richards Dawn P, Workman Jerry L, Hughes Timothy R, Greenblatt Jack, Coulombe Benoit
Abstract excerpt
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography allowed for the simple and efficient isolation of a complex containing all 12 RNAPII subunits, the general transcription factors TFIIB and TFIIF, the RNAPII phosphatase Fcp1, and a novel 153-kDa polypeptide of unknown...
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