A single nucleotide substitution in the 5'-untranslated region of the vaccinia N2L gene is responsible for both alpha-amanitin-resistant and temperature-sensitive phenotypes.

Abstract excerpt

The locus responsible for encoding resistance to alpha-amanitin was previously mapped to the vaccinia virus (VV) HindIII N fragment by using cloned wild-type VV DNA fragments to rescue the ability of an alpha-amanitin-resistant/temperature-sensitive VV mutant (alpha rts7) to replicate under nonpermissive conditions. DNA sequencing and transcriptional analyses of this region identified two leftward-reading open...

Topics

• Amanitins
• Base Sequence
• DNA, Viral
• Drug Resistance
• Genes, Viral
• Molecular Sequence Data
• Mutation
• Phenotype
• RNA, Messenger
• Restriction Mapping
• Temperature