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Article

Stopped-flow and mutational analysis of base flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase.

Journal of molecular biology - 6 Aug 2004

Liebert Kirsten, Hermann Andrea, Schlickenrieder Martina, Jeltsch Albert

Abstract excerpt

By stopped-flow kinetics using 2-aminopurine as a probe to detect base flipping, we show here that base flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase (MTase) is a biphasic process: target base flipping is very fast (k(flip)>240 s(-1)), but binding of the flipped base into the active site pocket of the enzyme is slow (k=0.1-2 s(-1)). Whereas base flipping occurs in the absence of...

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Share to Base Pair Mismatch
Share to Conserved Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA, Bacterial

Share to Escherichia coli

Share to Mutagenesis, Site-Directed

Share to S-Adenosylmethionine

Share to Sequence Homology, Amino Acid

Share to Site-Specific DNA-Methyltransferase (Adenine-Specific)

Source

Identifiers

PMID: 15276835
DOI: 10.1016/j.jmb.2004.05.033

Genes

GATC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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