Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase.

doi:10.1006/jmbi.1998.2389

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Article

Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase.

Journal of molecular biology - 22 Jan 1999

Jeltsch A, Roth M, Friedrich T

Abstract excerpt

DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely...

Topics

Cross-Linking Reagents
DNA
DNA Mutational Analysis
DNA-Binding Proteins
Mutagenesis, Site-Directed
Mutation
Oligodeoxyribonucleotides
Photolysis
Protein Binding
S-Adenosylmethionine
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Thermodynamics
Uracil

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