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Article

Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase.

Journal of molecular biology - 22 Jan 1999

Jeltsch A, Roth M, Friedrich T

Abstract excerpt

DNA methyltransferases flip their target base out of the DNA helix. Here, we have investigated base flipping by wild-type EcoRV DNA methyltransferase (M.EcoRV) and five M.EcoRV variants (D193A, Y196A, S229A, W231R and Y258A). These variants bind to DNA and S-adenosylmethionine but have a severely reduced catalytic efficiency or are catalytically inactive. To measure base flipping three different assays were used,...

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Source

Identifiers

PMID: 9918720
DOI: 10.1006/jmbi.1998.2389

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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