Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Identification of essential and non-essential genes of the guinea pig cytomegalovirus (GPCMV) genome via transposome mutagenesis of an infectious BAC clone.

Virus research - 1 May 2004

McGregor Alistair, Liu Fenyong, Schleiss Mark R

Abstract excerpt

We report application of a transposition methodology that allows the easy characterization and mutation of genes encoded on an infectious bacterial artificial chromosome (BAC) clone. We characterized mutants generated by transposome (Tn) mutagenesis of a BAC clone of guinea pig cytomegalovirus (GPCMV). A pool of Tn mutant GPCMV BACs were screened initially by restriction profile analysis to verify they were...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromosomes, Artificial, Bacterial
Share to DNA Fingerprinting

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Transposable Elements

Share to Genes, Essential

Share to Genes, Viral

Share to Genome, Viral

Share to Mutagenesis, Insertional

Share to Viral Structural Proteins

Source

Identifiers

PMID: 15041177
DOI: 10.1016/j.virusres.2003.12.030

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification of essential and non-essential genes of the guinea pig cytomegalovirus (GPCMV) genome via transposome mutagenesis of an infectious BAC clone.PMID 15041177Read ↗

Fast Screening Procedures for Random Transposon Libraries of Cloned Herpesvirus Genomes: Mutational Analysis of Human Cytomegalovirus Envelope Glycoprotein GenesSemantic proximity Read ↗

Analysis of the nucleotide sequence of the guinea pig cytomegalovirus (GPCMV) genomeSemantic proximity Read ↗

Genetic analysis of cytomegalovirus by shuttle mutagenesis.Semantic proximity Read ↗

A comprehensive functional landscape of PRV replication and pathogenesis revealed by genome-wide transposon mutagenesis.Semantic proximity Read ↗

Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replication.Semantic proximity Read ↗

Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/Cas9-Mediated Gene Editing.Semantic proximity Read ↗

A hyperactive piggyBac transposase for mammalian applications.Semantic proximity Read ↗

Identification of a 1.6 kb genome locus of guinea pig cytomegalovirus required for efficient viral growth in animals but not in cell cultureSemantic proximity Read ↗

Use of Bacterial Artificial Chromosomes in Generating Targeted Mutations in Human and Mouse CytomegalovirusesSemantic proximity Read ↗

Repair of a Mutation Disrupting the Guinea Pig Cytomegalovirus Pentameric Complex Acquired during Fibroblast Passage Restores Pathogenesis in Immune-Suppressed Guinea Pigs and in the Context of Congenital Infection.Semantic proximity Read ↗

Murine Cytomegalovirus m41 Open Reading Frame Encodes a Golgi-Localized Antiapoptotic ProteinSemantic proximity Read ↗

Functional map of human cytomegalovirus AD169 defined by global mutational analysis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.