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Article

A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

19 Sept 2013

Abstract excerpt

Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability...

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PMID: 24049164
DOI: 10.1128/jvi.02261-13

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