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Article

The isolation of strand-specific nicking endonucleases from a randomized SapI expression library.

Nucleic acids research - 1 Jan 2004

Samuelson James C, Zhu Zhenyu, Xu Shuang-yong

Abstract excerpt

The Type IIS restriction endonuclease SapI recognizes the DNA sequence 5'-GCTCTTC-3' (top strand by convention) and cleaves downstream (N1/N4) indicating top- and bottom-strand spacing, respectively. The asymmetric nature of DNA recognition presented the possibility that one, if not two, nicking variants might be created from SapI. To explore this possibility, two parallel selection procedures were designed to...

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Identifiers

PMID: 15247348
DOI: 10.1093/nar/gkh674
PMCID: PMC484165

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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