The nicking endonuclease N.BstNBI is closely related to type IIs restriction endonucleases MlyI and PleI.

doi:10.1093/nar/29.12.2492

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Article

The nicking endonuclease N.BstNBI is closely related to type IIs restriction endonucleases MlyI and PleI.

Nucleic acids research - 15 Jun 2001

Higgins L S, Besnier C, Kong H

Abstract excerpt

N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks the top strand preferentially. The Type IIs restriction endonucleases PleI and MlyI also recognize GAGTC, but cleave both DNA strands. Cloning and sequencing the genes encoding each of these three endonucleases discloses significant sequence similarities. Mutagenesis studies reveal a conserved set of catalytic residues among the three...

Topics

Amino Acid Sequence
Amino Acid Substitution
Base Sequence
Binding Sites
Calcium
Catalysis
Chromatography, Gel
Cloning, Molecular
Conserved Sequence
DNA
DNA-Binding Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Deoxyribonucleases, Type II Site-Specific

Dimerization

Geobacillus stearothermophilus

Molecular Sequence Data

Mutation

Oligodeoxyribonucleotides

Protein Binding

Source

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Identifiers

PMID: 11410656
DOI: 10.1093/nar/29.12.2492
PMCID: PMC55753

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