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Article

The nicking endonuclease N.BstNBI is closely related to type IIs restriction endonucleases MlyI and PleI.

Nucleic acids research - 15 Jun 2001

Higgins L S, Besnier C, Kong H

Abstract excerpt

N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks the top strand preferentially. The Type IIs restriction endonucleases PleI and MlyI also recognize GAGTC, but cleave both DNA strands. Cloning and sequencing the genes encoding each of these three endonucleases discloses significant sequence similarities. Mutagenesis studies reveal a conserved set of catalytic residues among the three...

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Identifiers

PMID: 11410656
DOI: 10.1093/nar/29.12.2492
PMCID: PMC55753

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