Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression and characterisation in E. coli of mutant forms of saporin.

Journal of biotechnology - 25 May 2005

Pittaluga Eugenia, Poma Anna, Tucci Adele, Spanò Laura

Abstract excerpt

In the present communication, we report on the expression and characterisation in Escherichia coli of mutant derivatives of saporin, a type 1 ribosome-inactivating protein from Saponaria officinalis L. The effects of substitution of Glu 176 with Lys and those of deletion of 19 amino acids at the C-terminal were evaluated both in vivo, testing the influence of expressed proteins on bacterial growth and in vitro...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Substitution
Share to Cell-Free System
Share to DNA, Superhelical

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Lysine

Share to N-Glycosyl Hydrolases

Share to Plant Proteins

Share to Recombinant Proteins

Share to Sequence Deletion

Source

Identifiers

PMID: 15862356
DOI: 10.1016/j.jbiotec.2005.01.021

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 9 closest

Expression and characterisation in E. coli of mutant forms of saporin.PMID 15862356Read ↗

The differential catalytic activity of ribosome-inactivating proteins saporin 5 and 6 is due to a single substitution at position 162.Semantic proximity Read ↗

N-terminal deletion affects catalytic activity of saporin toxin.Semantic proximity Read ↗

Maximization of dextransucrase activity expressed in E. coli by mutation and its functional characterization.Semantic proximity Read ↗

The isolation of strand-specific nicking endonucleases from a randomized SapI expression library.Semantic proximity Read ↗

Preparation and characterization of RNase P from Escherichia coli.Semantic proximity Read ↗

Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.Semantic proximity Read ↗

Functional characterization of the Staphylococcus carnosus SecA protein in Escherichia coli and Bacillus subtilis secA mutant strains.Semantic proximity Read ↗

Isolation and characterization of a Bacillus subtilis secA mutant allele conferring resistance to sodium azide.Semantic proximity Read ↗

A beta-turn rich barley seed protein is correctly folded in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.