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Article

Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity.

Journal of molecular biology - 7 Jun 2002

Samuelson James C, Xu Shuang-yong

Abstract excerpt

Restriction endonucleases have proven to be especially resistant to engineering altered substrate specificity, in part, due to the requirement of a cognate DNA methyltransferase for cellular DNA protection. The thermophilic restriction endonuclease BstYI recognizes and cleaves all hexanucleotide sequences described by 5'-R GATCY-3' (where R=A or G and Y=C or T). The recognition of a degenerate sequence is a...

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Identifiers

PMID: 12054862
DOI: 10.1016/S0022-2836(02)00343-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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