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Article

Novel hairpin-shaped primer assay to study the association of the -44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease.

Clinical and diagnostic laboratory immunology - 1 Jul 2004

Boniotto Michele, Hazbón Manzour Hernando, Jordan William James, Lennon Greig Patrick, Eskdale Joyce, Alland David, Gallagher Grant

Abstract excerpt

A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes and any post-PCR manipulation. The amplification is monitored in real-time using SYBR Green I dye and takes only 2 h...

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Source

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Identifiers

PMID: 15242954
DOI: 10.1128/CDLI.11.4.766-769.2004
PMCID: PMC440632

Genes

DEFB1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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