Article
Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV.
Yeast (Chichester, England) - 1 Jan 2000
Aalto M K, Ruohonen L, Hosono K, Keränen S
Abstract excerpt
The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature-sensitive mutation of sec1-1 at 37 degrees C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Glycoside Hydrolases
- Molecular Sequence Data
- Munc18 Proteins
- Mutation
- Nerve Tissue Proteins
- Open Reading Frames
- Plasmids
- RNA Splicing
- RNA, Fungal
- Restriction Mapping
- Saccharomyces cerevisiae