Article
Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV.
Yeast (Chichester, England) - 1 Jan 2000
Aalto M K, Ruohonen L, Hosono K, Keränen S
Abstract excerpt
The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature-sensitive mutation of sec1-1 at 37 degrees C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of 6.11. Hydrophobicity plotting showed no clearly hydrophobic regions suggesting a soluble nature for the protein. Amino...
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