Article
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system.
Plasmid - 1 Jan 2009
Hughes Stephen R, Sterner David E, Bischoff Kenneth M, Hector Ronald E, Dowd Patrick F, Qureshi Nasib, Bang Sookie S, Grynaviski Nicole, Chakrabarty Tania, Johnson Eric T, Dien Bruce S, Mertens Jeffrey A, Caughey Robert J, Liu Siqing, Butt Tauseef R, LaBaer Joshua, Cotta Michael A, Rich Joseph O
Abstract excerpt
A three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous yeast protease Ulp1 was developed for production of large amounts of soluble functional protein in Saccharomyces cerevisiae. Each vector has a different selectable marker (URA, TRP, or LEU), and the system provides high expression levels of three different proteins...
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