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Article

Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase.

The Journal of biological chemistry - 8 Oct 2004

Barabás Orsolya, Pongrácz Veronika, Kovári Júlia, Wilmanns Matthias, Vértessy Beáta G

Abstract excerpt

dUTPase is essential to keep uracil out of DNA. Crystal structures of substrate (dUTP and alpha,beta-imino-dUTP) and product complexes of wild type and mutant dUTPases were determined to reveal how an enzyme responsible for DNA integrity functions. A kinetic analysis of wild type and mutant dUTPases was performed to obtain relevant mechanistic information in solution. Substrate hydrolysis is shown to be initiated...

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Identifiers

PMID: 15208312
DOI: 10.1074/jbc.M406135200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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