Article
Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase.
Nucleic acids research - 1 Nov 2010
Pecsi Ildiko, Leveles Ibolya, Harmat Veronika, Vertessy Beata G, Toth Judit
Abstract excerpt
Aromatic interactions are well-known players in molecular recognition but their catalytic role in biological systems is less documented. Here, we report that a conserved aromatic stacking interaction between dUTPase and its nucleotide substrate largely contributes to the stabilization of the associative type transition state of the nucleotide hydrolysis reaction. The effect of the aromatic stacking on catalysis...
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