Article
Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis.
Archives of biochemistry and biophysics - 1 Oct 2009
Siggaard Julie H B, Johansson Eva, Vognsen Tina, Helt Signe Smedegaard, Harris Pernille, Larsen Sine, Willemoës Martin
Abstract excerpt
Two mutant dCTP deaminase-dUTPases from Methanocaldococcus jannaschii were crystallised and the crystal structures were solved: E145A in complex with the substrate analogue alpha,beta-imido-dUTP and E145Q in complex with diphosphate. Both mutant enzymes were defect in the deaminase reaction and had reduced dUTPase activity. In the structure of E145Q in complex with diphosphate, the diphosphate occupied the same...
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