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Article

Selection of Dictyostelium discoideum transformants and analysis of vector maintenance using live bacteria resistant to G418.

Plasmid - 1 Jul 1992

Hughes J E, Podgorski G J, Welker D L

Abstract excerpt

A protocol that allows the rapid isolation and growth of large numbers of independent G418-resistant Dictyostelium discoideum transformant colonies on the surface of agar media with live bacteria was developed. Transformants grown under these conditions form normal fruiting bodies. Discovery that aggregation of nontransformants was inhibited at a nonselective level of G418 (25 to 35 micrograms/ml) led to the...

Topics

Animals
Cell Division
DNA Replication
Dictyostelium
Extrachromosomal Inheritance
Genetic Variation
Genetic Vectors
Selection, Genetic
Transformation, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1518912
DOI: 10.1016/0147-619x(92)90035-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1518912-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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