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Article

Selection of Dictyostelium discoideum transformants and analysis of vector maintenance using live bacteria resistant to G418.

Plasmid - 1 Jul 1992

Hughes J E, Podgorski G J, Welker D L

Abstract excerpt

A protocol that allows the rapid isolation and growth of large numbers of independent G418-resistant Dictyostelium discoideum transformant colonies on the surface of agar media with live bacteria was developed. Transformants grown under these conditions form normal fruiting bodies. Discovery that aggregation of nontransformants was inhibited at a nonselective level of G418 (25 to 35 micrograms/ml) led to the...

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Source

Identifiers

PMID: 1518912
DOI: 10.1016/0147-619x(92)90035-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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