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Article

A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.

PloS one - 1 Jan 2015

Friedrich Michael, Meier Doreen, Schuster Isabelle, Nellen Wolfgang

Abstract excerpt

CHARACTERISTICS OF DIRS-1 MEDIATED KNOCK-DOWNS: We have previously shown that the most abundant Dictyostelium discoideum retroelement DIRS-1 is suppressed by RNAi mechanisms. Here we provide evidence that both inverted terminal repeats have strong promoter activity and that bidirectional expression apparently generates a substrate for Dicer. A cassette containing the inverted terminal repeats and a fragment of a...

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Identifiers

PMID: 26110905
DOI: 10.1371/journal.pone.0131271
PMCID: PMC4482531

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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