Quantification of transformation efficiency using a new method for clonal growth and selection of axenic Dictyostelium cells.

doi:10.1002/dvg.1020110513

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Article

Quantification of transformation efficiency using a new method for clonal growth and selection of axenic Dictyostelium cells.

Developmental genetics - 1 Jan 1990

Knecht D A, Jung J, Matthews L

Abstract excerpt

A new method for clonal growth of Dictyostelium axenic amoebae has been developed. Cells are plated in growth medium containing 1% ultra-low gelling temperature agarose. Cells grow normally in the agarose and form colonies up to several millimeters in diameter. When the colonies have grown to a s...

Topics

Agar
Blotting, Southern
Calcium Phosphates
Clone Cells
Culture Media
DNA, Fungal
Dictyostelium
Electricity
Genes, Fungal
Mutation
Transformation, Genetic

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