Article
Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.
Molecular & general genetics : MGG - 1 Mar 1991
Kalpaxis D, Zündorf I, Werner H, Reindl N, Boy-Marcotte E, Jacquet M, Dingermann T
Abstract excerpt
In the cellular slime mould Dictyostelium discoideum the two enzymatic activities of the pyrimidine pathway, orotidine-5'-phosphate decarboxylase (EC 4.1.1.23; OMPdecase) and orotate phosphoribosyl transferase (EC 2.4.2.10; OPRTase), are encoded by a single gene (DdPYR5-6). As in higher eukaryotes the bifunctional enzyme is referred to as UMP synthase. Here we present a method that allows efficient generation and...
Topics
- Blotting, Southern
- Dictyostelium
- Drug Resistance, Microbial
- Genes, Fungal
- Genotype
- Kinetics
- Multienzyme Complexes
- Mutation
- Orotate Phosphoribosyltransferase
- Orotic Acid