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Article

Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.

Molecular & general genetics : MGG - 1 Mar 1991

Kalpaxis D, Zündorf I, Werner H, Reindl N, Boy-Marcotte E, Jacquet M, Dingermann T

Abstract excerpt

In the cellular slime mould Dictyostelium discoideum the two enzymatic activities of the pyrimidine pathway, orotidine-5'-phosphate decarboxylase (EC 4.1.1.23; OMPdecase) and orotate phosphoribosyl transferase (EC 2.4.2.10; OPRTase), are encoded by a single gene (DdPYR5-6). As in higher eukaryotes the bifunctional enzyme is referred to as UMP synthase. Here we present a method that allows efficient generation and...

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PMID: 2017144
DOI: 10.1007/BF00261692

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