Article
A novel Dictyostelium discoideum gene required for cAMP-dependent cell aggregation.
Biochemical and biophysical research communications - 17 Mar 1998
Nagasaki A, Sutoh K, Adachi H, Sutoh K
Abstract excerpt
Using a method of random insertional mutagenesis called REMI (restriction enzyme-mediated integration), we isolated two mutant strains of Dictyostelium discoideum with a defect in cAMP-dependent cell aggregation. On bacterial lawns, both of the cells formed large and smooth plaques. When starved in a non-nutrient medium, they became elongated and extended pseudopods very frequently like starved wild type cells....
Topics
- Amino Acid Sequence
- Animals
- Chemotaxis
- Cloning, Molecular
- Cyclic AMP
- Dictyostelium
- Fungal Proteins
- Gene Expression
- Gene Targeting
- Genes, Fungal
- Genes, Protozoan
- Molecular Sequence Data
- Mutation
- Phenotype
- Protozoan Proteins
- Saccharomyces cerevisiae
- Schizosaccharomyces
- Sequence Homology, Amino Acid