Article
A simple method of generating axenic derivatives of Dictyostelium strains.
Experimental cell research - 1 Apr 1992
Morrison A, Harwood A
Abstract excerpt
We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer.
Topics
- Animals
- Dictyostelium
- Germ-Free Life
- Phenotype
- Plasmids