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Article

Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.

PloS one - 1 Jan 2018

Paschke Peggy, Knecht David A, Silale Augustinas, Traynor David, Williams Thomas D, Thomason Peter A, Insall Robert H, Chubb Jonathan R, Kay Robert R, Veltman Douwe M

Abstract excerpt

Dictyostelium has a mature technology for molecular-genetic manipulation based around transfection using several different selectable markers, marker re-cycling, homologous recombination and insertional mutagenesis, all supported by a well-annotated genome. However this technology is optimized for mutant, axenic cells that, unlike non-axenic wild type, can grow in liquid medium. There is a pressing need for...

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Identifiers

PMID: 29847546
DOI: 10.1371/journal.pone.0196809
PMCID: PMC5976153

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Source receipt: pubmed, retrieved 7 Sept 2026.

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