Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.

Nucleic acids research - 26 Oct 2004

Faix Jan, Kreppel Lisa, Shaulsky Gad, Schleicher Michael, Kimmel Alan R

Abstract excerpt

Dictyostelium discoideum has proven an exceptionally powerful system for studying numerous aspects of cellular and developmental functions. The relatively small ( approximately 34 Mb) chromosomal genome of Dictyostelium and high efficiency of targeted gene disruption have enabled researchers to characterize many specific gene functions. However, the number of selectable markers in Dictyostelium is restricted, as...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Dictyostelium
Share to Drug Resistance

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Targeting

Share to Genetic Markers

Share to Integrases

Share to Nucleosides

Share to Recombination, Genetic

Share to Viral Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 15507682
DOI: 10.1093/nar/gnh136
PMCID: PMC528815

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.PMID 15507682Read ↗

Generation of multiple knockout mutants using the Cre-loxP system.Semantic proximity Read ↗

Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination.Semantic proximity Read ↗

Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.Semantic proximity Read ↗

Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species.Semantic proximity Read ↗

Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker.Semantic proximity Read ↗

Dictyostelium discoideum as a Model for Investigating Neurodegenerative DiseasesSemantic proximity Read ↗

Using single loxP sites to enhance homologous recombination: ts mutants in Sec1 of Dictyostelium discoideum.Semantic proximity Read ↗

Rapid generation of gene disruption constructs by in vitro transposition and identification of a Dictyostelium protein kinase that regulates its rate of growth and development.Semantic proximity Read ↗

Mutation Screening of Dictyostelium Restriction Enzyme-Mediated Integration (REMI) Libraries.Semantic proximity Read ↗

An insertional mutagenesis approach to Dictyostelium cell death.Semantic proximity Read ↗

A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.Semantic proximity Read ↗

Dictyostelium discoideum as a non‐mammalian biomedical modelSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.