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Article

Uncoupling retro-translocation and degradation in the ER-associated degradation of a soluble protein.

The EMBO journal - 2 Jun 2004

Lee Robert J, Liu Chang-Wei, Harty Carol, McCracken Ardythe A, Latterich Martin, Römisch Karin, DeMartino George N, Thomas Philip J, Brodsky Jeffrey L

Abstract excerpt

Aberrant polypeptides in the endoplasmic reticulum (ER) are retro-translocated to the cytoplasm and degraded by the 26S proteasome via ER-associated degradation (ERAD). To begin to resolve the requirements for the retro-translocation and degradation steps during ERAD, a cell-free assay was used to investigate the contributions of specific factors in the yeast cytosol and in ER-derived microsomes during the ERAD...

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Identifiers

PMID: 15152188
DOI: 10.1038/sj.emboj.7600232
PMCID: PMC419910

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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