Article
Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles.
The Journal of cell biology - 1 Jul 1991
Rexach M F, Schekman R W
Abstract excerpt
The transport of pro-alpha-factor from the ER to the Golgi apparatus in gently lysed yeast spheroplasts is mediated by diffusible vesicles. These transport vesicles contain core-glycosylated pro-alpha-factor and are physically separable from donor ER and target Golgi compartments. The formation of diffusible vesicles from the ER requires ATP, Sec12p, Sec23p, and GTP hydrolysis. The vesicles produced are...
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