Article
Structural basis of the enhanced stability of a mutant ribozyme domain and a detailed view of RNA--solvent interactions.
Structure (London, England : 1993) - 7 Mar 2001
Juneau K, Podell E, Harrington D J, Cech T R
Abstract excerpt
BACKGROUND: The structure of P4-P6, a 160 nucleotide domain of the self-splicing Tetrahymena thermophila intron, was solved previously. Mutants of the P4-P6 RNA that form a more stable tertiary structure in solution were recently isolated by successive rounds of in vitro selection and amplification. RESULTS: We show that a single-site mutant (Delta C209) possessing greater tertiary stability than wild-type P4-P6...
Topics
- Animals
- Binding Sites
- Cobalt
- Crystallization
- Crystallography, X-Ray
- Ions
- Magnesium
- Manganese
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Protein Folding
- RNA, Catalytic
- Tetrahymena
- Water