Article
A rapid method for manual or automated purification of fluorescently labeled nucleic acids for sequencing, genotyping, and microarrays.
Journal of biomolecular techniques : JBT - 1 Mar 2003
Springer Amy L, Booth Lisa R, Braid Michael D, Houde Christiane M, Hughes Karin A, Kaiser Robert J, Pedrak Casandra, Spicer Douglas A, Stolyar Sergey
Abstract excerpt
Fluorescent dyes provide specific, sensitive, and multiplexed detection of nucleic acids. To maximize sensitivity, fluorescently labeled reaction products (e.g., cycle sequencing or primer extension products) must be purified away from residual dye-labeled precursors. Successful high-throughput analyses require that this purification be reliable, rapid, and amenable to automation. Common methods for purifying...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
