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Article

The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.

Journal of molecular biology - 30 Apr 2021

Schmidt Thomas G M, Eichinger Andreas, Schneider Markus, Bonet Lidia, Carl Uwe, Karthaus Dennis, Theobald Ina, Skerra Arne

Abstract excerpt

The affinity system based on the artificial peptide ligand Strep-tag® II and engineered tetrameric streptavidin, known as Strep-Tactin®, offers attractive applications for the study of recombinant proteins, from detection and purification to functional immobilization. To further improve binding of the Strep-tag II to streptavidin we have subjected two protruding loops that shape its ligand pocket for the peptide...

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Crystallography, X-Ray
Ligands
Mutagenesis
Peptides
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Engineering

Recombinant Fusion Proteins

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PMID: 33639211
DOI: 10.1016/j.jmb.2021.166893

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