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Article

Generation of readily transformable Bacillus licheniformis mutants.

Applied microbiology and biotechnology - 1 Feb 2008

Waschkau Bianca, Waldeck Jens, Wieland Susanne, Eichstädt Renèe, Meinhardt Friedhelm

Abstract excerpt

A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids isolated from Escherichia coli was routinely possible when the double mutant B. licheniformis...

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PMID: 18046550
DOI: 10.1007/s00253-007-1278-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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