Article
A technique for integrating any DNA fragment into the chromosome of Escherichia coli.
Gene - 1 Jan 2000
Raibaud O, Mock M, Schwartz M
Abstract excerpt
We describe a technique that allows the insertion of any DNA fragment into the EcoRI-site-containing malPpa, the promoter of malPQ, one of the three maltose operons of Escherichia coli. DNA fragments were cloned into the unique EcoRI site of the pBR322-derived plasmid pOM40, which carries malPpa. In the next step these fragments were transposed into the chromosome by homologous recombination events occurring on...
Topics
- Base Sequence
- Chromosomes, Bacterial
- DNA Restriction Enzymes
- DNA Transposable Elements
- DNA, Recombinant
- Escherichia coli
- Genes
- Genes, Bacterial
- Genotype
- Maltose
- Operon
- Plasmids