Article
Specific gene silencing in the pre-implantation stage mouse embryo by an siRNA expression vector system.
Molecular reproduction and development - 1 May 2004
Haraguchi Seiki, Saga Yumiko, Naito Kunihiko, Inoue Hirokazu, Seto Akira
Abstract excerpt
Recently, small interfering RNAs (siRNAs) have become a powerful and widely used tool for the analysis of gene function in mammalian cells. Here we report that the microinjection of an siRNA expression vector into the nucleus is an efficient and powerful method of specific gene silencing in pre-implantation mouse embryos. We used this method to examine the expression of two genes EGFP and Oct4. Vectors encoding...
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