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Article

A transient transgenic RNAi strategy for rapid characterization of gene function during embryonic development.

PloS one - 16 Dec 2010

Bjork Bryan C, Fujiwara Yuko, Davis Shannon W, Qiu Haiyan, Saunders Thomas L, Sandy Peter, Orkin Stuart, Camper Sally A, Beier David R

Abstract excerpt

RNA interference (RNAi) is a powerful strategy for studying the phenotypic consequences of reduced gene expression levels in model systems. To develop a method for the rapid characterization of the developmental consequences of gene dysregulation, we tested the use of RNAi for "transient transgenic" knockdown of mRNA in mouse embryos. These methods included lentiviral infection as well as transposition using the...

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Identifiers

PMID: 21179568
DOI: 10.1371/journal.pone.0014375
PMCID: PMC3002952

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Source receipt: pubmed, retrieved 7 Sept 2026.

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