Article
RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos.
The Journal of reproduction and development - 1 Jan 2013
Oikawa Mami, Matoba Shogo, Inoue Kimiko, Kamimura Satoshi, Hirose Michiko, Ogonuki Narumi, Shiura Hirosuke, Sugimoto Michihiko, Abe Kuniya, Ishino Fumitoshi, Ogura Atsuo
Abstract excerpt
In mice, one of the major epigenetic errors associated with somatic cell nuclear transfer (SCNT) is ectopic expression of Xist during the preimplantation period in both sexes. We found that this aberrant Xist expression could be impeded by deletion of Xist from the putative active X chromosome in donor cells. In male clones, it was also found that prior injection of Xist-specific siRNA could significantly improve...
Topics
- Alleles
- Animals
- Cell Nucleus
- Cloning, Organism
- Cumulus Cells
- Epigenesis, Genetic
- Female
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Hydroxamic Acids
- In Situ Hybridization, Fluorescence
- Male