Article
[Regulation of [12Asp]K-ras4B on transcriptional activity of estrogen receptor in endometrial carcinoma HEC-1A cell lines].
Zhonghua fu chan ke za zhi - 1 Jan 2004
Gui Li-ming, Wei Li-hui, Xu Ming-xu, Wang Jian-liu, Zhong Ying-cheng, Li Xiao-ping, Tu Zheng, Sun Peng-ming, Ma Da-long
Abstract excerpt
OBJECTIVE: To investigate the effect of mutant-type [(12)Asp]K-ras4B gene on the expression of estrogen receptor (ER) alpha and beta and their transcriptional activity as a transcription factor in endometrial carcinoma HEC-1A cell line. METHODS: (1) Effect of [(12)Asp]K-ras4B on the expression of ER alpha and beta were determined using Western blot assay. (2) Eukaryotic expression plasmid pGL3-luciferase-ERE...
Topics
- Animals
- Blotting, Western
- Cell Line, Tumor
- Endometrial Neoplasms
- Estrogen Receptor alpha
- Estrogen Receptor beta
- Female
- Humans
- Mice
- Mutation
- NIH 3T3 Cells
- Proto-Oncogene Proteins p21(ras)
- Receptors, Estrogen
- Transcription, Genetic