Phosphorylation of the recombinant spliced variants of the alpha-sub-unit of the stimulatory guanine-nucleotide binding regulatory protein (Gs) by the catalytic sub-unit of protein kinase A.

doi:10.1016/0006-291x(92)90857-h

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Article

Phosphorylation of the recombinant spliced variants of the alpha-sub-unit of the stimulatory guanine-nucleotide binding regulatory protein (Gs) by the catalytic sub-unit of protein kinase A.

Biochemical and biophysical research communications - 31 Jul 1992

Pyne N J, Freissmuth M, Pyne S

Abstract excerpt

Both GS alpha-1 and GS alpha-4 were phosphorylated by the purified catalytic sub-unit of protein kinase A. Phosphate incorporation into 220 pmol and 190 pmol of GS alpha-4 and GS alpha-1 after a 1 hour incubation with kinase was 14 pmol and 10 pmol, respectively. These low levels of phosphorylation are due to the thermal lability of purified recombinant GS alpha. However, the phosphorylation was inhibited by...

Topics

Autoradiography
Brain
Electrophoresis, Polyacrylamide Gel
Escherichia coli
GTP-Binding Proteins
Genetic Variation
Guanosine Diphosphate
Guanosine Triphosphate
Guanylyl Imidodiphosphate
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetics

Macromolecular Substances

Molecular Weight

Phosphorus Radioisotopes

Protein Kinases

RNA Precursors

RNA Splicing

Recombinant Proteins

Source

Open in PubMed

Identifiers

PMID: 1497643
DOI: 10.1016/0006-291x(92)90857-h

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1497643-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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