Article
Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Specific activation of Gsalpha by an NDPK B.Gbetagamma complex in H10 cells.
The Journal of biological chemistry - 28 Feb 2003
Hippe Hans-Joerg, Lutz Susanne, Cuello Friederike, Knorr Katrin, Vogt Achim, Jakobs Karl H, Wieland Thomas, Niroomand Feraydoon
Abstract excerpt
Formation of GTP by nucleoside diphosphate kinase (NDPK) can contribute to G protein activation in vitro. To study the effect of NDPK on G protein activity in living cells, the NDPK isoforms A and B were stably expressed in H10 cells, a cell line derived from neonatal rat cardiomyocytes. Overexpression of either NDPK isoform had no effect on cellular GTP and ATP levels, basal cAMP levels, basal adenylyl cyclase...
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