Article
Human tumor p53 mutations are selected for in mouse embryonic fibroblasts harboring a humanized p53 gene.
Proceedings of the National Academy of Sciences of the United States of America - 2 Mar 2004
Liu Zhipei, Hergenhahn Manfred, Schmeiser Heinz H, Wogan Gerald N, Hong Amanda, Hollstein Monica
Abstract excerpt
To date, there has been no way to examine induced human p53 gene mutations in cell cultures exposed to mutagenic factors, other than by restriction site analysis. Here, we used embryonic cells from our Hupki (human p53 knock-in) mouse strain to generate human p53 DNA-binding domain (DBD) mutations experimentally. Twenty cultures of untreated primary mouse Hupki fibroblasts and 20 short-wavelength UV light...
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