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Article

A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements.

Nucleic acids research - 18 Feb 2004

Yang Allen S, Estécio Marcos R H, Doshi Ketan, Kondo Yutaka, Tajara Eloiza H, Issa Jean-Pierre J

Abstract excerpt

We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic loci, could be used for direct sequencing, selective restriction digestion or pyrosequencing, in order to quantitate...

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Identifiers

PMID: 14973332
DOI: 10.1093/nar/gnh032
PMCID: PMC373427

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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