Article
Isolation of new ribozymes from a large pool of random sequences [see comment].
Science (New York, N.Y.) - 10 Sept 1993
Bartel D P, Szostak J W
Abstract excerpt
An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate two RNA molecules that are aligned on a template by catalyzing the attack of a 3'-hydroxyl on an adjacent 5'-triphosphate--a reaction similar to that employed by the familiar protein enzymes that synthesize RNA. The corresponding...
Topics
- Base Sequence
- Biological Evolution
- Catalysis
- Kinetics
- Magnesium
- Molecular Sequence Data
- Mutation
- Oligoribonucleotides
- RNA
- RNA Ligase (ATP)
- RNA, Catalytic
- Temperature