Article
SmD1 is required for spliced leader RNA biogenesis.
Eukaryotic cell - 1 Feb 2004
Zeiner Gusti M, Foldynová Silvie, Sturm Nancy R, Lukes Julius, Campbell David A
Abstract excerpt
The Sm-binding site of the kinetoplastid spliced leader RNA has been implicated in accurate spliced leader RNA maturation and trans-splicing competence. In Trypanosoma brucei, RNA interference-mediated knockdown of SmD1 caused defects in spliced leader RNA maturation, displaying aberrant 3'-end formation, partial formation of cap 4, and overaccumulation in the cytoplasm; U28 pseudouridylation was unaffected.
Topics
- Alternative Splicing
- Animals
- Binding Sites
- Cytoplasm
- DNA, Kinetoplast
- Exons
- Mutation
- Phenotype
- Protein Binding
- Pseudouridine
- RNA
- RNA Interference