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Article

Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA.

Nucleic acids research - 1 Jun 2010

Shaked Hadassa, Wachtel Chaim, Tulinski Pawel, Yahia Nasreen Hag, Barda Omer, Darzynkiewicz Edward, Nilsen Timothy W, Michaeli Shulamit

Abstract excerpt

In trypanosomes a 39 nucleotide exon, the spliced leader (SL) is donated to all mRNAs from a small RNA, the SL RNA, by trans-splicing. Since the discovery of trans-splicing in trypanosomes two decades ago, numerous attempts failed to reconstitute the reaction in vitro. In this study, a crude whole-cell extract utilizing the endogenous SL RNA and synthetic tubulin pre-mRNA were used to reconstitute the...

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Identifiers

PMID: 20159996
DOI: 10.1093/nar/gkq065
PMCID: PMC2879531

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Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA.PMID 20159996Read ↗

<i>Trans</i> -Splicing in Trypanosomes: Machinery and Its Impact on the Parasite TranscriptomeSemantic proximity Read ↗

The Pre-mRNA Splicing Machinery of Trypanosomes: Complex or Simplified?Semantic proximity Read ↗

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Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements.Semantic proximity Read ↗

A trans-spliced telomerase RNA dictates telomere synthesis in Trypanosoma brucei.Semantic proximity Read ↗

Trypanosomal TBP Functions with the Multisubunit Transcription Factor tSNAP To Direct Spliced-Leader RNA Gene ExpressionSemantic proximity Read ↗

Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIHSemantic proximity Read ↗

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